BIOPRINTING NEWS

3D-BIOPRINTED TISSUES CAN BE STORED IN THE FREEZER UNTIL THE NEED! The short shelf life of 3D tissues limits their clinical use. In the case of organ transplantation, the tissue produced by the bioprinting method must be transported quickly to the place where it is needed. Otherwise, the 3D-bioprinted tissue will lose its vitality. Researchers have published a study combining 3D bioprinting and cryopreservation technique to create tissues that can be stored in a -196°C freezer and defrosted within minutes for use when needed. Biomedical engineer Y. Shrike Zhang says that there is no shelf life in the traditional bioprinting method, 3D tissues are used after bioprinting. With the cryobioprinting method, you can bioprint as much as you want and store the products in a frozen state. The bioink is flowed through the nozzle to create the defined shape, allowing the structure to form layer by layer. Bioink consists of a gelatin-like scaffold in which living cells are embedded. In cryobioprin

  CUTTING EDGE TECHNOLOGY TO BIOPRINT MINI-KIDNEYS!  Using 3D bioprinting technology, the researchers produced miniature human kidneys in the lab. The study includes biotech company Organovo, Murdoch Children's Research Institute (MCRI). The research team also validated the use of 3D bioprinted human mini-kidneys to screen for drug toxicity from a class of drugs known to cause kidney damage in humans. The study demonstrated how 3-D bioprinting of stem cells would produce sheets of kidney tissue large enough for transplants. Artificial living tissue was produced using extrusion-based 3D bioprinting technology and bioink consisting of stem cells. Melissa Little, who started growing kidney organoids in 2015, stated that the 3D bioprinting method allows a faster and more reliable process. The study found that with 3D bioprinting, it was able to create about 200 mini-kidneys in 10 minutes without sacrificing quality. The mini-kidneys produced resemble a normal-sized kidney. Using mini-o

3D BIOPRINTING TECHNIQUE CONTROLS CELL ORIENTATION!  Using 3D bioprinting technology, tissue scaffolds that mimic tissues can be printed. Controlling cellular organization in these scaffolds designed for tissue engineering studies is a complex and challenging process. Produced cellular scaffolds must perform like natural tissues. To achieve this, cells must have a regular structure in terms of spatial distribution and alignment. In this study, cellular orientation was controlled by the technique developed for fast, simple and cost-effective printing of multi-compartment hydrogel fibers. Alginate and GELMA hydrogel fibers were printed in the determined pattern with the static mixer integrated into the coaxial microfluidic system. In the engineered microstructure, GelMA chambers provide a suitable environment for the cell, while alginate chambers offer morphological and mechanical properties that guide cellular orientation. It has been demonstrated in this study that cellular alignment c

NOSE CARTRIDGE PRINTED WITH 3D BIOPRINTING TECHNOLOGY! A team of University of Alberta researchers has produced a specially shaped cartilage u sing three-dimensional bioprinting technology  that can be used in surgical applications. The hydrogel prepared with cells taken from the patient was used in bioprinting. Surgeons reshape the cartilage taken from the patient's ribs to fit the size and shape required for reconstructive surgery and transplant the patient. With this traditional procedure, however, there is a risk of complications in the patient. The other problem is that the rib compartment that protects the lungs needs to be opened to reconstruct the nose. This region has a vital anatomical importance. The patient's lungs may have collapsed. Researchers say that this study is an example of regenerative medicine. The cartilage, which is specially printed for the patient and grown in the laboratory, has the potential to eliminate the risk of collapse in the lungs, infection

RESEARCHERS USED A NEW TECHNOLOGY FOR BIOPRINTING ADULT NEURON CELLS! A group of researchers has managed to maintain a high level of cell viability and functionality by using laser-assisted bioprinting technology to print adult neuron cells. The method known as laser-induced side-transfer (LIST) enables three-dimensional bioprinting with higher efficiency by improving existing bioprinting techniques using bioinks with different viscosities. In the study, dorsal root ganglion (DRG) neurons from the peripheral nervous system of mice were used. Neurons suspended in bioink were loaded into a square capillary located on a biocompatible substrate. Low-energy laser pulses focused on the midpoint of the capillary, printing the cell-loaded bioink in droplets. Figure 1. Bioprinting system After bioprinting, different tests were applied. In the viability test, it was concluded that 86 percent of the cells survived two days after printing. The researchers note that when using low-energy laser puls