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3D BIOPRINTING TECHNIQUE CONTROLS CELL ORIENTATION! 


Using 3D bioprinting technology, tissue scaffolds that mimic tissues can be printed. Controlling cellular organization in these scaffolds designed for tissue engineering studies is a complex and challenging process. Produced cellular scaffolds must perform like natural tissues. To achieve this, cells must have a regular structure in terms of spatial distribution and alignment.

In this study, cellular orientation was controlled by the technique developed for fast, simple and cost-effective printing of multi-compartment hydrogel fibers. Alginate and GELMA hydrogel fibers were printed in the determined pattern with the static mixer integrated into the coaxial microfluidic system. In the engineered microstructure, GelMA chambers provide a suitable environment for the cell, while alginate chambers offer morphological and mechanical properties that guide cellular orientation. It has been demonstrated in this study that cellular alignment can be adapted by controlling the flow parameters during the printing process.

Extrusion-based bioprinting technology is the most widely used bioprinting method. In extrusion-based bioprinting, the hydrogel fibers are typically several hundred micrometers in size with randomly oriented cells. Consequently, a technique is required that provides topographic cues to cells within the printed fibers to orient their organization.

According to the team, scaffolds produced by extrusion-based 3D bioprinting method should consist of very thin filaments in order to guide the cellular organization.

Ali Tamayol stated that "this bioprinting technique enables the generation of morphological features of tissue structures - with a resolution up to dimensions comparable to the size of cells - to control cellular behavior and create biomimetic structures. It shows great potential for engineering fibrillar tissues such as skeletal muscles, tendons and ligaments." also expressed.

Figure 1. Biofabrication of multicompartmental hydrogel fibers



REFERENCES

Controlling cellular organization in bioprinting through designed 3D microcompartmentalization

3D bioprinting technique controls cell orientation

Mohamadmahdi Samandari

Fatemeh Alipanah

Keivan Majidzadeh-A

Grissel Trujillo de Santiago

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